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AIM: To investigate the differences of myopia and ocular biological parameters of primary and middle school students in Urumqi.METHODS: A cross-sectional survey. A total of 2 495 primary and middle school students aged 7 to 18 from 4 schools in Urumqi were selected by judgemental sampling from September 2021 to November 2021 for relevant eye examination. The differences of ocular biological parameters and refractive status of students of different ages, genders and nationalities were compared, and the correlation between spherical equivalent(SE)and ocular biological parameters was analyzed.RESULTS: The detection rate of poor vision among the students was 80.36%, the detection rate of myopia was 55.91%, and the detection rate of astigmatism was 42.96%, among which the detection rate of low myopia was 63.80%, the detection rate of moderate myopia was 27.60%, and the detection rate of high myopia was 8.60%. There were significant differences in the detection rate of poor vision, myopia, astigmatism, SE and some ocular biological parameters among students of different ages and nationalities(all P<0.05). Among them, the detection rate of myopia, astigmatism and poor vision among Uygur and Kazakh ethnic groups was significantly lower than that of Han. The detection rate of poor vision and myopia among boys was lower than that among girls, while the detection rate of astigmatism was higher than that of girls. Spearman correlation analysis showed that axis length of eye, axial length to axial ratio, anterior chamber depth, and pupil diameter were negatively correlated with SE(rs=-0.664, -0.724, -0.320, -0.086, all P<0.001), and lens thickness was positively correlated with SE(rs=0.147, P<0.001).CONCLUSION: The prevalence of myopia among primary and secondary school students in Urumqi is high, and there are differences in the distribution of ocular biological parameters among children and adolescents of different ages and ethnicities.
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The polymyxins are important antimicrobial agents against antibiotic-resistant gram-negative bacilli. In 2020, the Clinical and Laboratory Standards Institute modified the clinical breakpoints for polymyxin susceptibility test by eliminating the "susceptible" interpretive category, only reporting intermediate (≤2 mg/L) and resistant (≥4 mg/L). However, the European Committee on Antimicrobial Susceptibility Testing recommended the use of clinical breakpoints of ≤2 mg/L as susceptible and >2 mg/L as resistant. The first-line laboratorians and clinicians in China have been perplexed by the inconsistence of international polymyxin clinical breakpoints and discouraged by the difficulty of conducting polymyxin susceptibility testing. Therefore, it is urgently needed to make it clear for the laboratorians in China to know how to accurately carry out polymyxin susceptibility testing and standardize the interpretation of susceptibility testing results. To this end, the experts from relevant fields were convened to formulate this consensus statement on the testing and clinical interpretation of polymyxin susceptibility. Relevant recommendations are proposed accordingly for laboratorians and clinicians to streamline their daily work.
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@#AIM: To analyze the correlation and agreement of corneal horizontal diameter(WTW)measured with caliper, IOL Master and Oculyzer anterior segment analysis system.<p>METHODS: Totally 41 patients with 80 eyes who underwent ICL V4c implantation in our hospital from July 2018 to January 2019 were enrolled. Compare the correlation and agreement of preoperative WTW measured with caliper, IOL Master and Oculyzer anterior segment analysis system respectively.<p>RESULTS: The mean WTW value measured with caliper, IOL Master and Oculyzer anterior segment analysis system were: 11.73±0.40, 12.03±0.46, 11.54±0.39mm respectively, the value of caliper and IOL Master, caliper and Oculyzer, IOL Master and Oculyzer were highly correlated(<i>r</i>=0.809, 0.826, 0.785, all <i>P</i><0.01), and the 95% <i>LoA</i> of Bland-Altman analysis were(-0.84, 0.22),(-0.27, 0.64),(-0.07, 1.05)mm respectively.<p>CONCLUSION: Among the three measuring instruments, IOL Master has the largest measurement value and Oculyzer is the smallest. The three measurement methods have poor consistency and cannot be replaced in clinical application. Whereas, their measurements are highly correlated. In clinical application, the measurements of IOL Master and Oculyzer can be corrected based on the difference with caliper.
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Objective There are few studies on the in vitro induction of human leukemia cell line THP-1 macrophages by Staphylococcus aureus PV leukin S component (LukS-PV). This study aims to investigate whether LukS-PV could induce the polarization of Human THP-1 derived macrophage in vitro. Methods THP-1 monocytes were induced to macrophages by Phorbol myristate acetate (PMA). The cell surface markers CD11b and CD14 were detected by flow cytometry at 48h and 72h after the induction, respectively. Using 0.1, 0.2 and 0.4 μmol/LlukS-PV treated THP-1 macrophages as the 0.1 μmol/LLukS-PV group, 0.2 μmol/LLukS-PV group, and 0.4 μmol/LLukS-PV group, respectively. The THP-1 driven macrophage induced by PMA was used as the control group. Flow cytometry was used to detect the expression of CD80 and CD206 in each group at 24h and 48h. The mRNA expression levels of IL-12, TNF-α and TGF-β were detected by real-time fluorescence-based quantitative PCR (qRT-PCR). Results The expression of CD80 on the cell surface in the 0.1 μmol/LLukS-PV group, 0.2 μmol/LLukS-PV group and 0.4 μmol/LLukS-PV significantly increased (P <0.01), with the highest expression in the 0.2 μM LukS-PV group (P<0.01), when comparing to that of the control group. The mRNA expressions of IL-12 and TNF-α in the 0.2 μM LukS-PV group significantly increased (7.32±0.91 and 5.29±0.51, respectively) at 24h (P <0.01), and raised in 0.2 μM LukS-PV group (11.16±0.78、6.70±0.68) at 48h (P <0.01), when comparing to those of control group (0.71±0.11 and 0.73±0.14, respectively). Compared with the control group, expression of CD80 on cell surface in the 0.2 μM LukS-PV group significantly increased at 24h and 48h (P <0.000). Conclusion LukS-PV could stimulate the polarization of human THP-1 macrophages toward M1.
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Fourteen chemical constituents, including 5-hydroxy-4-methoxy-1-tetralone(1), 4,8-dihydroxy-1-tetralone(2), 4,5-dihydroxy-α-tetralone(3), blumenol B(4), dehydrovomifoliol(5), megastigm-5-ene-3,9-diol(6), juglanin B(7), blumenol C(8), loliolide(9), oleracone B(10), syringarsinol(11), pinoresinol(12), methyl 4-hydroxy-3-methoxybenzoate(13), and isovanillic acid(14), were isolated from the dichloromethane fraction of 95% methanol extract of green walnut husks by silica gel and MCI column chromatography, and Pre-HPLC. Their structures were determined by spectroscopic methods, such as NMR, MS and so on. Among them, compounds 1, 4-6, 8-13 were isolated from the green walnut husks for the first time, and compounds 4-6, 8, 10, 12, 13 were isolated from the Juglans genus for the first time. All of isolates were detected their inhibitory activities against HeLa, HGC-27 and Ht-29 cell lines by the MTT assay. The result showed that compounds 2, 3, 7, 9 and 11 exhibited inhibitory activity against the tested cell line. The IC_(50) of 7 were 26.5, 9.0, 25.4 μmol·L~(-1), respectively.